The occurrence of chronic non-cancer pain (CNCP) has seen a substantial rise over the past few decades. This, coupled with the widespread and unchecked utilization of prescribed opioids, has emerged as a significant concern for public health. While there is some evidence, it is limited, regarding the possibility of endocrine dysfunction as a consequence of long-term opioid treatment (L-TOT). This study seeks to explore the connections between L-TOT and various endocrine markers in patients suffering from CNCP.